abstract：:The cellular role of Hsp100/Clp chaperones in maintaining protein stability is based on two functional aspects. Under normal growth conditions they represent components of cellular protein quality control machineries that selectively remove damaged or misfolded polypeptides in cooperation with specific proteases. Afte...

journal_title：Journal of structural biology

authors： Leidhold C,von Janowsky B,Becker D,Bender T,Voos W

更新日期：2006-10-01 00:00:00

abstract：:The dyneins are a family of microtubule motor proteins. The motor domain, which represents the C-terminal 2/3 of the dynein heavy chain, exhibits homology to the AAA family of ATPases. It consists of a ring of six related but divergent AAA+ units, with two substantial sized protruding projections, the stem, or tail, w...

journal_title：Journal of structural biology

authors： Vallee RB,Höök P

更新日期：2006-10-01 00:00:00

abstract：:The AAA family proteins usually form a hexameric ring structure. The ATP-binding pocket, which is located at the interface of subunits in the hexamer, consists of three functionally important motifs, the Walker A and B motifs, and the second region of homology (SRH). It is well known that Walker A and B motifs mediate...

journal_title：Journal of structural biology

authors： Yakushiji Y,Nishikori S,Yamanaka K,Ogura T

更新日期：2006-10-01 00:00:00

abstract：:Two membrane-bound ATP-dependent AAA proteases conduct protein quality surveillance in the inner membrane of mitochondria and control crucial steps during mitochondrial biogenesis. AAA domains of proteolytic subunits are critical for the recognition of non-native membrane proteins which are extracted from the membrane...

journal_title：Journal of structural biology

authors： Graef M,Langer T

更新日期：2006-10-01 00:00:00

abstract：:Charge contrast imaging (CCI) is a dynamic phenomenon recently reported in insulating and semiconducting materials imaged with low vacuum or variable pressure scanning electron microscopes (SEM). Data presented in this paper illustrates that CCI can also be applied to biominerals and biological soft-tissues and that u...

journal_title：Journal of structural biology

authors： Clode PL

更新日期：2006-09-01 00:00:00

abstract：:Titin is a giant protein responsible for passive-tension generation in muscle sarcomeres. Here, we used single-molecule AFM force spectroscopy to investigate the mechanical characteristics of a recombinant construct from the human cardiac-specific N2B-region, which harbors a 572-residue unique sequence flanked by two ...

journal_title：Journal of structural biology

authors： Leake MC,Grützner A,Krüger M,Linke WA

更新日期：2006-08-01 00:00:00

abstract：:The properties and characteristics shared by amyloid fibrils formed from disease and non-disease associated proteins that are unrelated in sequence and structure offer the prospect that model systems can be used to systematically assess the factors that predispose a native protein to form amyloid fibrils. Based on a d...

journal_title：Journal of structural biology

authors： Kammerer RA,Steinmetz MO

更新日期：2006-08-01 00:00:00

abstract：:Null-mutation in Drosophila importin-alpha2, such as the deficiency imp-alpha2(D14), causes recessive female sterility with the formation of dumpless eggs. In imp-alpha2(D14) the transfer of nurse cell components to the oocyte is interrupted and the Kelch protein, an oligomeric ring canal actin organizer, is normally ...

journal_title：Journal of structural biology

authors： Gorjánácz M,Török I,Pomozi I,Garab G,Szlanka T,Kiss I,Mechler BM

更新日期：2006-04-01 00:00:00

abstract：:We present a model-based parallel algorithm for origin and orientation refinement for 3D reconstruction in cryoTEM. The algorithm is based upon the Projection Theorem of the Fourier Transform. Rather than projecting the current 3D model and searching for the best match between an experimental view and the calculated p...

journal_title：Journal of structural biology

authors： Ji Y,Marinescu DC,Zhang W,Zhang X,Yan X,Baker TS

更新日期：2006-04-01 00:00:00

abstract：:The morphology and structure of giant starch granules from the pseudo-bulbs of Phajus grandifolius were investigated, using a number of microscopy techniques together with synchrotron radiation microdiffration analysis. Most of the granules, which had sizes between 100 and 200 microm, occurred as ogival particles with...

journal_title：Journal of structural biology

authors： Chanzy H,Putaux JL,Dupeyre D,Davies R,Burghammer M,Montanari S,Riekel C

更新日期：2006-04-01 00:00:00

abstract：:In situ hybridization (ISH) is a widely applied technique used for visualizing specific nucleic acid sequences at chromosomal, cytologic, and histologic levels. It sometimes fails, however, to demonstrate precise cell identity, early stages of gene expression and variants of alternative splicing because of its limited...

journal_title：Journal of structural biology

authors： Kitazawa S,Kitazawa R

更新日期：2006-01-01 00:00:00

abstract：:The sulfur-oxidizing enzyme system (Sox) of the chemotroph Paracoccus pantotrophus is composed of several proteins, which together oxidize hydrogen sulfide, sulfur, thiosulfate or sulfite and transfers the gained electrons to the respiratory chain. The hetero-dimeric cytochrome c complex SoxXA functions as heme enzyme...

journal_title：Journal of structural biology

authors： Dambe T,Quentmeier A,Rother D,Friedrich C,Scheidig AJ

更新日期：2005-12-01 00:00:00

abstract：:To characterize the spatial and temporal occurrence of programmed cell death (PCD) in Lilium anther tissues, we used both microscopical and molecular markers of apoptosis for developmental stages from meiosis to pollen release. The first hallmarks of PCD include cell condensation and shrinkage of the cytoplasm, separa...

journal_title：Journal of structural biology

authors： Varnier AL,Mazeyrat-Gourbeyre F,Sangwan RS,Clément C

更新日期：2005-11-01 00:00:00

abstract：:In fishes, like in amphibians, it is well established that variations in rRNA activity occur during oogenesis. Contrary to amphibians, however, little is known about the ultrastructural changes of the nucleolus during fish oogenesis. Evolution of the nucleolus has been followed during oogenesis in the teleost fish Bar...

journal_title：Journal of structural biology

authors： Thiry M,Poncin P

更新日期：2005-10-01 00:00:00

abstract：:Crustaceans form clots by the rapid crosslinking of a hemolymph clottable protein (CP) to form long, branched polymers. Clotting limits hemolymph loss from wounds as well as playing a part in the innate immune response. CP is a 420 kDa homodimer with a large quantity of associated lipid, primarily the carotenoid pigme...

journal_title：Journal of structural biology

authors： Kollman JM,Quispe J

更新日期：2005-09-01 00:00:00

abstract：:DNA regions close to the origin of replication were visualized by the green fluorescent protein (GFP)-Lac repressor/lac operator system. The number of oriC-GFP fluorescent spots per cell and per nucleoid in batch-cultured cells corresponded to the theoretical DNA replication pattern. A similar pattern was observed in ...

journal_title：Journal of structural biology

authors： Elmore S,Müller M,Vischer N,Odijk T,Woldringh CL

更新日期：2005-09-01 00:00:00

abstract：:We report here on the current state of our efforts in automated molecular microscopy. Our primary automated data acquisition software system, Leginon, has been completely redesigned over the past two years. The new distributed system has been developed using the Python programming language and is compatible with both ...

journal_title：Journal of structural biology

authors： Suloway C,Pulokas J,Fellmann D,Cheng A,Guerra F,Quispe J,Stagg S,Potter CS,Carragher B

更新日期：2005-07-01 00:00:00

abstract：:It has been known since the 19th century that coral skeletons are built of aragonite crystals with taxonomy-linked arrangements, but the way by which each coral species controls this crystallization process remains an unsolved question. The problem became still more intriguing when it was shown that isotopic compositi...

journal_title：Journal of structural biology

authors： Cuif JP,Dauphin Y

更新日期：2005-06-01 00:00:00

abstract：:Formation of filaments by the Ure2 protein constitutes the molecular mechanism of the [URE3] prion in yeast. According to the "amyloid backbone" model, the N-terminal asparagine-rich domains of Ure2p polymerize to form an amyloid core fibril that is surrounded by C-terminal domains in their native conformation. Protea...

journal_title：Journal of structural biology

authors： Baxa U,Cheng N,Winkler DC,Chiu TK,Davies DR,Sharma D,Inouye H,Kirschner DA,Wickner RB,Steven AC

更新日期：2005-05-01 00:00:00

abstract：:The Dugesia japonica vasa-like gene B (DjVLGB) protein is a DEAD-box RNA helicase of a planarian, which is well known for its strong regenerative capacity. DjVLGB shares sequence similarity to the Drosophila germ-line-specific DEAD-box RNA helicase Vasa, and even higher similarity to its paralogue, mouse PL10. In this...

journal_title：Journal of structural biology

authors： Kurimoto K,Muto Y,Obayashi N,Terada T,Shirouzu M,Yabuki T,Aoki M,Seki E,Matsuda T,Kigawa T,Okumura H,Tanaka A,Shibata N,Kashikawa M,Agata K,Yokoyama S

更新日期：2005-04-01 00:00:00

abstract：:This is the first crystal structure of a carbohydrate induced dimer of phospholipase A(2) (PLA(2)). This is an endogenous complex formed between two PLA(2) molecules and two mannoses. It was isolated from Krait venom (Bungarus caeruleus) and crystallized as such. The complete amino acid sequence of PLA(2) was determin...

journal_title：Journal of structural biology

authors： Singh G,Gourinath S,Sarvanan K,Sharma S,Bhanumathi S,Betzel Ch,Yadav S,Srinivasan A,Singh TP

更新日期：2005-03-01 00:00:00

abstract：:The biomechanical properties of the zone of calcified cartilage (ZCC) in articulating joints are of clinical relevance due to the role ZCC plays in load transfer from cartilage to bone. To determine the micron-level mechanical properties and their correlation to mineral concentration in the ZCC, we combined nanoindent...

journal_title：Journal of structural biology

authors： Gupta HS,Schratter S,Tesch W,Roschger P,Berzlanovich A,Schoeberl T,Klaushofer K,Fratzl P

更新日期：2005-02-01 00:00:00

abstract：:We have used conical tomography to study the structure of integral proteins in their phospholipid bilayer environments. Complete conical series were collected from replicas of the water channel aquaporin-0 (AQP0), a 6.6 nm side tetramer with a molecular weight of approximately 120 kDa that was purified and reconstitut...

journal_title：Journal of structural biology

authors： Lanzavecchia S,Cantele F,Bellon PL,Zampighi L,Kreman M,Wright E,Zampighi GA

更新日期：2005-01-01 00:00:00

abstract：:Neuropeptide gamma (NPgamma) is a neurokinin-2 (NK-2) receptor selective agonist, which plays an important role in mediation of asthma and elicits a wide range of biological responses like bronchoconstriction, vasodepression and regulation of endocrine functions. The structure determination of this peptide agonist is ...

journal_title：Journal of structural biology

authors： Chandrashekar IR,Dike A,Cowsik SM

更新日期：2004-12-01 00:00:00

abstract：:Zn-alpha2-glycoprotein (ZAG) is a 41 kDa soluble protein that is present in most bodily fluids. The previously reported 2.8 A crystal structure of ZAG isolated from human serum demonstrated the structural similarity between ZAG and class I major histocompatibility complex (MHC) molecules and revealed a non-peptidic li...

journal_title：Journal of structural biology

authors： Delker SL,West AP Jr,McDermott L,Kennedy MW,Bjorkman PJ

更新日期：2004-11-01 00:00:00

abstract：:The crystal structure of the YckF protein from Bacillus subtilis was determined with MAD phasing and refined at 1.95A resolution. YckF forms a tight tetramer both in crystals and in solution. Conservation of such oligomerization in other phosphate sugar isomerases indicates that the crystallographically observed tetra...

journal_title：Journal of structural biology

authors： Sanishvili R,Wu R,Kim DE,Watson JD,Collart F,Joachimiak A

更新日期：2004-10-01 00:00:00

abstract：:A new method for the flexible fitting of high-resolution structures into low-resolution maps of macromolecular complexes from electron microscopy has been recently described in applications to simulated electron density maps. This method uses a linear combination of low-frequency normal modes in an iterative manner to...

journal_title：Journal of structural biology

authors： Tama F,Miyashita O,Brooks CL 3rd

更新日期：2004-09-01 00:00:00

abstract：:Sub-nanometer resolution structure determination is becoming a common practice in electron cryomicroscopy of macromolecular assemblies. The data for these studies have until now been collected on photographic film. Using cytoplasmic polyhedrosis virus (CPV), a previously determined structure, as a test specimen, we sh...

journal_title：Journal of structural biology

authors： Booth CR,Jiang W,Baker ML,Zhou ZH,Ludtke SJ,Chiu W

更新日期：2004-08-01 00:00:00

abstract：:Third harmonic generation microscopy is shown to be a robust method for obtaining structural information on a variety of biological specimens. Its nature allows depth-resolved imaging of inhomogeneities with virtually no background from surrounding homogeneous media. With an appropriate illumination geometry, third ha...

journal_title：Journal of structural biology

authors： Oron D,Yelin D,Tal E,Raz S,Fachima R,Silberberg Y

更新日期：2004-07-01 00:00:00

abstract：:The mechanisms which cause macromolecules to form discrete compartments within the nucleus are not understood. Here, two ubiquitous compartments, nucleoli, and PML bodies, are shown to disassemble when K562 cell nuclei expand in medium of low monovalent cation concentration; their major proteins dispersed as seen by i...

journal_title：Journal of structural biology

authors： Hancock R

更新日期：2004-06-01 00:00:00

abstract：:Lichens are symbiotic associations of fungi and algae (or cyanobacteria). Fungal cells produce large amounts of lipid, assisted by algae, and secrete them out of the cells. Some of the secreted lipids crystallize in the thallus of the lichen. The crystalline materials sometimes occupy 30% of total dry weight of the th...

journal_title：Journal of structural biology

authors： Arakawa-Kobayashi S,Kobayashi T,Hasebe M,Kanaseki T

更新日期：2004-06-01 00:00:00

abstract：:AAA+ proteins remodel target substrates in an ATP-dependent manner, an activity that is of central importance for a plethora of cellular processes. While sharing a similar hexameric structure AAA+ proteins must exhibit differences in substrate recognition to fulfil their diverse biological functions. Here we describe ...

journal_title：Journal of structural biology

authors： Mogk A,Dougan D,Weibezahn J,Schlieker C,Turgay K,Bukau B

更新日期：2004-04-01 00:00:00

abstract：:The AAA+ family of proteins play fundamental roles in all three kingdoms of life. It is thought that they act as molecular chaperones in aiding the assembly or disassembly of proteins or protein complexes. Recent structural studies on a number of AAA+ family proteins have revealed that they share similar structural el...

journal_title：Journal of structural biology

authors： Smith GR,Contreras-Moreira B,Zhang X,Bates PA

更新日期：2004-04-01 00:00:00

abstract：:Hyaline cartilage consists of sparse chondrocytes and abundant extracellular matrix. There is a paucity of experimental data in support of the notion of conceivable regional differences in the mechanical properties of chondral matrices. Upon visual differentiation of the pericellular and interterritorial matrices in e...

journal_title：Journal of structural biology

authors： Allen DM,Mao JJ

更新日期：2004-03-01 00:00:00

abstract：:DNA-dependent protein kinase (DNA-PK) is part of the eukaryotic DNA double strand break repair pathway and as such is crucial for maintenance of genomic stability, as well as for V(D)J (variable-diversity-joining) recombination. The catalytic subunit of DNA-PK (DNA-PKcs) belongs to the phosphatidylinositol-3 (PI-3) ki...

journal_title：Journal of structural biology

authors： Brewerton SC,Doré AS,Drake AC,Leuther KK,Blundell TL

更新日期：2004-03-01 00:00:00

abstract：:Over recent years advances in cryo-electron microscopy for the study of macromolecular structure have resulted in resolutions in the range 10-15 A becoming routine. With this drive for increased resolution comes the need to collect larger datasets, commonly >10,000 particle images. Manual selection of particles from m...

journal_title：Journal of structural biology

authors： Hall RJ,Patwardhan A

更新日期：2004-01-01 00:00:00

abstract：:Three-dimensional reconstruction of large macromolecules like viruses at resolutions below 10 A requires a large set of projection images. Several automatic and semi-automatic particle detection algorithms have been developed along the years. Here we present a general technique designed to automatically identify the p...

journal_title：Journal of structural biology

authors： Singh V,Marinescu DC,Baker TS

更新日期：2004-01-01 00:00:00

abstract：:OxlT is a bacterial transporter protein with 12 transmembrane segments that belongs to the Major Facilitator Superfamily of transporters. It facilitates the exchange of oxalate and formate across the membrane of the Gram-negative bacterium Oxalobacter formigenes. From an electron crystallographic analysis of two-dimen...

journal_title：Journal of structural biology

authors： Heymann JA,Hirai T,Shi D,Subramaniam S

更新日期：2003-12-01 00:00:00

abstract：:A common technique in transmission electron microscopy is the collection of a focal pair, in which the first, close to focus image contains higher resolution information at lower contrast, and the second, far from focus image has high contrast but less reliable high-resolution information. Typically these second micro...

journal_title：Journal of structural biology

authors： Ludtke SJ,Chiu W

更新日期：2003-10-01 00:00:00

abstract：:We describe here a set of procedures and algorithms that may be used as an aid in determining the indexing rule of a helical specimen. Crystallizing macromolecules into helical arrays has the potential to speed up and simplify the process of three-dimensional reconstruction of the macromolecular structure. The process...

journal_title：Journal of structural biology

authors： Ward A,Moody MF,Sheehan B,Milligan RA,Carragher B

更新日期：2003-10-01 00:00:00